What Are Molecular Techniques In Microbiology?


Molecular techniques used in microbiological diagnosis include nucleic acid amplification techniques, DNA sequencing and typing, direct molecular probe techniques (in situ hybridization), and nucleic acid quantitation.



What are molecular techniques in microbiology?

Molecular techniques used in microbiological diagnosis include nucleic acid amplification techniques, DNA sequencing and typing, direct molecular probe techniques (in situ hybridization), and nucleic acid quantitation.


Which of the following techniques can serve the purpose of early diagnosis of disease?

Complete answer:

Recombinant DNA technology or R-DNA technology puts together DNA molecules from two different organisms to create new genetic combinations and are introduced into the host organism. It may provide reliable information on genetic disorders for diagnosis purposes.


How is DNA used in genetic engineering?

Genetic engineering, also called recombinant DNA technology, involves the group of techniques used to cut up and join together genetic material, especially DNA from different biological species, and to introduce the resulting hybrid DNA into an organism in order to form new combinations of heritable genetic material.


What are the techniques used in genetic engineering?

Commonly used methods include:

  • microinjection of DNA into the nucleus of anchored cells;
  • electroporation, where DNA is introduced through cell membrane pores by pulsed electrical charges;
  • polycationic neutralization of the cell membrane and the DNA to be introduced to improve passive uptake;


What does early diagnosis mean?

Early diagnosis of cancer focuses on detecting symptomatic patients as early as possible so they have the best chance for successful treatment.


What do you mean by molecular diagnosis?

Listen to pronunciation. (muh-LEH-kyoo-ler dy-ug-NOH-sis) The process of identifying a disease by studying molecules, such as proteins, DNA, and RNA, in a tissue or fluid.


What is molecular diagnosis of genetic diseases?

Molecular diagnostic methods refer to the new genre of tests that detect abnormalities at the DNA level. Because genetic mutations are defined as heritable changes of an individual's DNA, these tests represent the most direct approach in assessing whether a patient is carrying a particular mutation.


What techniques are used for early genetic diagnosis?

So, the correct answer is 'R-DNA technology'.


Why is early diagnosis important?

An early diagnosis opens the door to future care and treatment. It helps people to plan ahead while they are still able to make important decisions on their care and support needs and on financial and legal matters.


What are the techniques that used for molecular diagnosis of viruses?

Table 1.

TechniqueFormatExamples of viruses
Target amplification techniquesMultiplex PCRHerpesviruses, respiratory viruses
Real-time PCRMost of viruses
Transcriptional-based amplification methodsHCV (TMA-based), HIV (TMAbased, NASBA), CMV (NASBA) Enterovirus (NASBA), RSV (NASBA)
Strand displacement amplificationHIV


Why is Molecular Medicine important in early disease diagnosis?

A branch of medicine that develops ways to diagnose and treat disease by understanding the way genes, proteins, and other cellular molecules work. Molecular medicine is based on research that shows how certain genes, molecules, and cellular functions may become abnormal in diseases such as cancer.


Why molecular tests are used as diagnostic tests?

Molecular diagnostics, such as multiplex real-time PCR, have replaced cell culture, fluorescent antigen-antibody detection, and antigen detection methodologies because they have high sensitivity and specificity, excellent quality control procedures, and detect a large battery of viral and bacterial pathogens rapidly in


Which molecular diagnostic technique is very useful for early detection of disease?

Polymerase chain reaction (PCR): The various forms of PCR are the most frequently used molecular diagnostic techniques in the diagnosis of infectious pathogens.


What techniques are used in molecular diagnosis?

These molecular diagnostic techniques include molecular cloning, macromolecule blotting and probing, gel electrophoresis, polymerase chain reaction, fluorescent in situ hybridization, spectral karyotyping imaging and DNA microarrays.


Which is used for early diagnosis?

Reason: Recombinant DNA technology, PCR and ELISA are some of the techniques that serve the purpose of early diagnosis.


Dated : 18-Jul-2022

Category : Education

Leave Your Comment